Most clogs are caused by salt precipitate build up or non clarified samples within the sample probe.
1. Remove the sample probe and sonicate the narrow end and flush with distilled water using a narrow gauge syringe. Replace the sample probe and verify the probe height.
2. Then perform the following maintenance wash -
3 backflushes, 3 drains, 2 alcohol washes and 3 washes with water (or use the maintenance wash script) Attempt to Calibrate.
If calibration fails:
Open the door in the middle of the front of the LX100/200. Disconnect the blue peak tubing that is screwed into the silver cylinder under the optical bench (black box) - shown on the left in the image below . Place the end of the peak tubing into a microfuge tube and place paper towels under the optics platform where the tubing was disconnected. Run a wash and watch for fluid in three places: waste container, peak tubing and under the optics platform.
Use the table below to identify the location of the clog. Repeat steps 1 & 2.
| SYMPTOMS | LOCATION |
||
| Fluid Dripping to Waste? | Fluid Dripping from Peak Tubing? | Fluid Dripping from Optics Platform? | |
|---|---|---|---|
| YES | NO, VERY LITTLE | YES | Clog in peak tubing. Reconnect peak tubing by pushing the tubing through the fitting and into the optics platform and then tightening the fitting. Run a wash and check for leaks around the connections. Attempt calibration and look for dripping from the sample probe. |
| NO | YES | NO | Clog in cuvette . Reconnect peak tubing by pushing the tubing through the fitting and into the optics platform and then tightening the fitting. Run a wash and check for leaks around the connections. Attempt calibration and look for dripping from the sample probe. |
| YES | YES | NO | Clog in sample injection needle of optics assembly. Reconnect peak tubing by pushing the tubing through the fitting and into the optics platform and then tightening the fitting. Run a wash and check for leaks around the connections. Attempt calibration and look for dripping from the sample probe. |
| YES | YES | YES | Potential problem with valve contact your instrument service representative. |
