Technote #
21 All bead events are missing their target region on the bead map
Date Updated Category
17-08-07 Data Acquisition
[STarStation 2.0][STarStation 2.3]

There are several potential causes for this phenomenon.

1) Air bubbles in system.

2) Low sheath buffer volume or sheath pressure.

3) Increase in sheath pressure due to an over-full waste container.

4) An incompatible solvent has been used during assay preparation and the assay microsphere colour codes have been affected.

5) The classification laser is misaligned.

6) The classifier dyes in the assay micropsheres have been damaged by exposure to light or freezing/thawing.

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