Applied Cytometry

Reagents & Consumables

» STarUtility Plates
» xMAP Sheath Fluid
» Calibration Microspheres
» Control Microspheres
» Multi-Analyte Microspheres
» FlexMAP™ Microspheres

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STarUtility Plates



STarUtility plates are designed for use with STarStation 2.3. The specialised plates allow you to automate daily maintenance procedures and give true walk away operation.

xMAP Sheath Fluid

Luminex Sheath Fluid is intended for use as the delivery medium of the sample to the optics component. It is available in a 20L format or a 20X concentrate that offers the benefits of:

  • Save space
  • Reduce waste
  • Reduce shipping cost

» Download MSDS [PDF]

Calibration Microspheres®

5mL xMAP® Classification Calibration Microspheres®
Microspheres of known fluorescent light intensities used to calibrate the settings for DD, CL1 and CL2 channels for the Luminex 200 and the Luminex 100 IS systems.

5mL xMAP® Reporter Calibration Microspheres®
Microspheres of known fluorescent light intensities used to calibrate the settings for the RP1 channel for the Luminex 200 and the Luminex 100 IS systems.

» Download MSDS [PDF]

Control Microspheres®

5 ml xMAP® Classification Control Microspheres®
Microspheres that are used to verify the calibration and optical integrity of the DD, CL1 and CL2 channels of the Luminex system.

5 ml xMAP® Reporter Control Microspheres®
Microspheres that are used to verify the calibration and optical integrity for the reporter channel for the Luminex system.

» Download MSDS [PDF]

xMAP® Multi-Analyte COOH Microspheres
Microspheres are general use components for further manufacture of xMAP bioassays. They are internally labelled with fluorescent dyes and contain surface Carboxyl groups for covalent attachment of ligands (or biomolecules). They are at a concentration of 1.25x10E7 beads/ml.

» Download MSDS [PDF]

xMAP® Multi-Analyte LumAvidin Microspheres
Microspheres are general use components for further manufacture of xMAP bioassays. They are internally labelled with fluorescent dyes and contain a surface layer of Avidin for near covalent binding of biotin and biotinylated ligands. They are at a concentration of 2.5x10E6 beads/ml.

» Download MSDS [PDF]

FlexMAP™ Beads

FlexMAP™ beads are fluorescent microspheres comprising 100 unique colour codes enabling individual beads to be identified using a Luminex® STarSystem.

A single strand of DNA - unique to each bead colour code - is bound to a designated microsphere, which enables complementary custom oligonucleotide primers to bind to their corresponding bead. The oligonucleotide primers are biotinylated, enabling their quantification with a Luminex® STarSystem.

This allows you to multiplex nucleotide assays – dramatically improving the rate in which you can analyse the results of PCR runs. This is ideal for high throughput analysis of SNPs, DNA and RNA, with minimal contamination and maximum accuracy.

  • PCR fragments are generated using custom-tagged oligonucleotides - up to 100 from one sample.
  • PCR products are bound to a colour-coded FlexMAP™ bead. FlexMAP™ bead bound PCR products are stained with fluorescent phycoerythrin.
  • The FlexMAP™ bead and fluorescent tag are analysed using a Luminex® STarSystem to quantify the concentration of PCR product bound to each individual bead.
  • Data is analysed easily using STarBase nucleotide analysis software.

FlexMAP™ beads, from Applied Cytometry, combined with Luminex® technology give you the potential to analyse the products of 100 PCR reactions, per well, from a 96 well plate. Use FlexMAP™ beads to analyse the products of 9600 PCR runs in under two hours.



» Download MSDS [PDF]

For more information contact customersupport@appliedcytometry.com



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