Applied Cytometry

VenturiOne™ FAQ's

1. The Plot Format and Region Format tabs are not displayed in the VenturiOne™ Ribbon?


2. I cannot visualize the Quadrant statistics displayed on the Preview plots. How can I make them clearer?


3. The distribution of events is not clearly displayed on the Preview plots. How do I optimally display the data?


4. What is V-log scaling?


5. Why does the system slow down when my gate equation contains 30 Boolean operands?


6. Why are the compensation values not displayed on all of the plots?


7. I cannot manually alter the compensation of the preview plots.


8. When Linear scaling is selected why does the compensation matrix display unexpected values?


9. What is a compensation file?


10. The compensation settings are not as expected when I open an LMD file?


11. The calculated compensation value, displayed on the compensation matrix, is greyed out when it should be represented in a black and bold format.


12. What is the Parameter Resolver?


13. What is included in a VenturiOne™ Workspace?


14. I have created a region but cannot visualize the region name and associated statistical value?


15. The created region and associated statistic is not displayed on the plot.


16. A known population of events is no longer displayed in the colour precedence plot.


17. A colour precedence plot displays a black population.


18. Multiple Quadrant regions have been applied to a plot, how can I distinguish which names and statistics are associated to each individual Quadrant?


19. Is it possible to create polygonal re-entrants?


20. Why can I not use the Region Position button (Region Format tab) to modify the position of a created Polygonal region?


21. I have created multiple regions on a plot, but cannot distinguish the names and associated statistical values because they overlie each other. How can I view this data?


22. I have manually re-positioned a region name. How can I now return it to its original position?


23. What is ChartJunk?


24. How can I display the column header menu when there are no column headers displayed in the results table?


25. When multiple column headers are added to the group control, the table indent hides the results, how can I visualize these results?


26. The entered gate equation exceeds the limits of the gate equation dialog box.


27. If the desktop colour theme is modified in Windows XP, why is the VenturiOne™ theme not changed?



1. The Plot Format and Region Format tabs are not displayed in the VenturiOne™ Ribbon?

The Plot Format tab is only displayed when a plot is selected in the Plots area. The Region Format tab is only displayed when a region is selected on a plot displayed in the Plots area.

2. I cannot visualize the Quadrant statistics displayed on the Preview plots. How can I make them clearer?

Click the Increase Preview Plot Size button (Preview tab) to magnify all the plots displayed in the Previews area.

3. The distribution of events is not clearly displayed on the Preview plots. How do I optimally display the data?

Change the scaling of the plots to optimally display the events population. Select either Linear, Logarithmic or V-log from the Preview tab.

4. What is V-log scaling?

V-Log is an axis transformation that is similar to Logarithmic for the upper three decades, but has a Linear section around the axis. This allows events that have a ‘negative’ fluorescence value to be better visualized on the plots.

5. Why does the system slow down when my gate equation contains 30 Boolean operands?

If a gate equation contains more than 23 Boolean operands (i.e. regions), the system may slow down significantly. We recommend that you limit the number of operands to 23.


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6. Why are the compensation values not displayed on all of the plots?

Compensation values are only displayed on the dual parameter plots that are displayed in the Previews area.


7. I cannot manually alter the compensation of the preview plots.

Ensure that the Manual Compensation option (Preview tab) is selected.

8. When Linear scaling is selected why does the compensation matrix display unexpected values?

A compensation matrix can only be calculated accurately when Log or V-Log scaling is selected, we do not recommend using Linear scaling when calculating a compensation matrix.

9. What is a compensation file?

Fluorescence compensation settings that are applied to a Workspace can be saved as a compensation file and applied to other compensatable files.

10. The compensation settings are not as expected when I open an LMD file?

Ensure that the Carry Compensation option (Preview tab) is not checked.

11. The calculated compensation value, displayed on the compensation matrix, is greyed out when it should be represented in a black and bold format.

After the software calculates the compensation coefficients for a single-stained file, values that do not require any compensation (i.e. represented as 0%)will still appear grey even though they have been calculated.


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12. What is the Parameter Resolver?

If the parameters in a file to be opened do not match those selected in the current Plots area, a Parameter Resolver is displayed. This enables the matching of the parameters so the new file can be opened in the current workspace.

13. What is included in a VenturiOne™ Workspace?

Plots, regions, gates, color precedence, selected statistics and displayed parameters are included in a VenturiOne™ Workspace.

14. I have created a region but cannot visualize the region name and associated statistical value?

The region label must fit within the boundaries of the plot space. Ensure that the region name is within these limits and that the associated statistic is displayed with the minimal number of decimal places.

15. The created region and associated statistic is not displayed on the plot.

Check that the designated region colour is not white. A white region and text will be hidden by the white plot background.

16. A known population of events is no longer displayed in the colour precedence plot.

Check that the colour white is not selected as a colour precedence option. The white population will be hidden by the white plot background.

17. A colour precedence plot displays a black population.

Check that black is not selected as a colour precedence option. Alternatively, ensure that the relevant gate is checked in the Precedence Order dialog.

18. Multiple Quadrant regions have been applied to a plot, how can I distinguish which names and statistics are associated to each individual Quadrant?

Hover the cursor over the intercept of a Quadrant to display a tool tip displaying the region names and associated statistics.

19. Is it possible to create polygonal re-entrants?

Single and multiple vertical re-entrants can be created.

20. Why can I not use the Region Position button (Region Format tab) to modify the position of a created Polygonal region?

The Region Position button can only be used to modify the position of a created Elliptical, Rectangular, Linear or Quadrant region.

21. I have created multiple regions on a plot, but cannot distinguish the names and associated statistical values because they overlie each other. How can I view this data?

Region names can be repositioned by selecting a region and dragging the name to a new position, as indicated by the cursor.

22. I have manually re-positioned a region name. How can I now return it to its original position?

Select the region and click the Automatic Region Name Position button (Region Format tab). The associated region name will return to its default position.


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23. What is ChartJunk?

ChartJunk are the details displayed on the exported images; Background Shading, Regions, Gate Names, Region Labels, Axis Labels, Plot Frame and Axes Tickmarks.

24. How can I display the column header menu when there are no column headers displayed in the results table?

If all the column headers are un-checked, it is not possible to right click on a column header to display the pop-up menu. To return this functionality, check a statistic check box (e.g. Count) positioned in the Statistics tab. The statistic is then displayed in the table. Right click on this column header to display the pop-up menu (refer to section 3.9.6).

25. When multiple column headers are added to the group control, the table indent hides the results, how can I visualize these results?

Double click the column separator for the hidden column to optimally visualize the column data.

26. The entered gate equation exceeds the limits of the gate equation dialog box.

If the entered Gate Equation exceeds the limits of the dialog box, click the button (Edit Gates dialog) to display a larger Gate Equation dialog box. Type the gate equation in this box (refer to section 3.5.6.1).

27. If the desktop colour theme is modified in Windows XP, why is the VenturiOne™ theme not changed?

The colour theme is only modified in VenturiOne™ when using Windows Vista.

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