Spectral Flow Cytometry

What is spectral flow cytometry?

As the name suggests, there are exciting optical features to spectral flow cytometry analysis which give the data more uses.

Spectral flow cytometry uses spectral detection to capture the emission spectrum of each fluorescent molecule. 

 

What was the need that made spectral flow cytometry arise?

Spectral overlap meant that with conventional flow cytometry, you couldn’t see fluorochromes as close together, and the number of colours were quite limited. 

There was a significant amount of data that couldn’t be used because of this, and scientists recognised the potential information this data could bring.

 

What are the real world applications for spectral cytometry?

The creation of spectral flow cytometry means that we can now study immune responses in more detail through defining more cellular parameters concurrently in single cells in a higher resolution than in traditional flow cytometry.

 

How has analysis changed due to spectral flow cytometry?

All the detectors in the flow cytometer collect the full emission spectra of each fluorophore, which makes it easy to discern slight differences between cells with similar signatures. 
Spectral unmixing is done through mathematics, and can’t be done manually, which has been something that flow cytometry software providers have worked on to make it as simple as possible.

 

VenturiOne – FCS Analysis

Spectral unmixing in flow cytometry needs to be done before your FCS files are analysed. You can then analyse your FCS data in VenturiOne.

You can trial our software for 30 days for free, and find out how your Spectral Flow Cytometry projects 

 

Read more:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556726/

https://www.thermofisher.com/uk/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-learning-center/flow-cytometry-resource-library/flow-cytometry-methods/spectral-flow-cytometry-fundamentals.html

https://www.frontiersin.org/articles/10.3389/fmolb.2020.612801/full

https://www.sonybiotechnology.com/us/blog/introduction-to-spectral-flow-cytometry/

https://voices.uchicago.edu/ucflow/spectral-flow-cytometry

 

 

 

 

 

AI in flow cytometry research