Schedule the processing of multiple folders of FCS data sequentially, and export your statistics to CSV files. Make your high dimensional processing just that bit faster with our flow data analysis tutorials.
Welcome to this Commander tutorial, this tutorial has been made to introduce you to Commander and help you get started using the software.
Let’s look at the commander software, within the commander software we can choose what clients we want to use.
A client is a host PC that will run the VenturiOne analysis software.
You can have as many clients as you want to set up, and the system will spread the analysis
across all the different clients that are connected.
To specify a client click the add button, and enter a name for that client as well as the IP address for that client.
It is better for this solution if the IP address remains static.
The client PC must be running VenturiOne software, and if the system can connect to VenturiOne.
You will see a green tick if for any reason the system can’t connect to a client you will see the red spyglass indicating that it can’t see a copy of VenturiOne.
Above that we have the folders area, where the folders containing the data files for analysis are.
So here what I can do is add one or more files ready for analysis, in this case I’m going to add this folder here.
Within the settings I need to set up various locations.
Firstly the location and the name of the playlist I want to use to analyze my files.
So the first workspace within that playlist will be the workspace that all the files within the particular location are analyzed within the base folder.
This is simply the starting point that I use to search when I’m looking for the folders of data
so when you select the add button it will take you to this location.
The shared folder location is where temporary playlists are constructed for all of the files that need analyzing.
The shared location must be a location that the clients have access to as well as the commander software.
The results folder is the location where your exported results will be stored.
This will be in the form of a CSV file.
Per plate analyzed, all of these network locations must be visible to the client PC, and the Commander PC.
If you use a location that is not visible, the software will not work.
You have added the folders of data files to begin analysis we need to press the start button the software will create a temporary playlist and send that playlist to a client for processing.
So here we have a copy of VenturiOne running alongside Commander, and in practice you will probably have VenturiOne running on a separately networked PC.
Once I press start the files are analyzed, and the results gathered double-clicking on a completed item in the Commander dialog will allow you to see the completed results in the csv file.
Here we see that the spreadsheet shows the results from all the regions from all the plots on all of the samples.
That was a small experiment, so let’s give it some more data to actually work through.
I will delete the files that i have already run and I’m going to add in four folders of 384 well plate data
I’ll select the playlist template that I want to use and then start processing.
Once the plates are completed, now you can see that the results are all saved in the results folder that we actually pointed to and that is the commander software.
If you would like any more information, please visit the product here.